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pH-nCas9-PPE-V2
(Plasmid #170131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHUE411 (modified from pCambia)
  • Backbone manufacturer
    Qi-Jun Chen
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nCas9(H840A)-M-MLV
  • Alt name
    NLS-nCas9(H840A)-NLS-32aa linker-M-MLV-NLS
  • Species
    M. musculus (mouse); Streptococcus pyogenes
  • Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L603W for M-MLV
  • Promoter maize Ubiquitin-1, OsU3

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH-nCas9-PPE-V2 was a gift from Caixia Gao (Addgene plasmid # 170131 ; http://n2t.net/addgene:170131 ; RRID:Addgene_170131)

  • For your References section:

    High-efficiency prime editing with optimized, paired pegRNAs in plants. Lin Q, Jin S, Zong Y, Yu H, Zhu Z, Liu G, Kou L, Wang Y, Qiu JL, Li J, Gao C. Nat Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 10.1038/s41587-021-00868-w. 10.1038/s41587-021-00868-w PubMed 33767395
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