pSpG-PPE
(Plasmid
#170130)
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PurposeFor plant prime editing in wheat plants or monocotyledons protoplasts
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenCas9-SpG(H840A)-M-MLV
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Alt nameNLS-nCas9-SpG(H840A)-NLS-32aa linker-M-MLV-NLS
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SpeciesM. musculus (mouse); Streptococcus pyogenes
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MutationH840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R for Cas9; D200N, T306K, W313F, T330P and L603W for M-MLV
- Promoter maize Ubiquitin-1
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSpG-PPE was a gift from Caixia Gao (Addgene plasmid # 170130 ; http://n2t.net/addgene:170130 ; RRID:Addgene_170130) -
For your References section:
High-efficiency prime editing with optimized, paired pegRNAs in plants. Lin Q, Jin S, Zong Y, Yu H, Zhu Z, Liu G, Kou L, Wang Y, Qiu JL, Li J, Gao C. Nat Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 10.1038/s41587-021-00868-w. 10.1038/s41587-021-00868-w PubMed 33767395