pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D
(Plasmid #169912)

• Purpose: Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.
• Depositing Lab: Zhili Rong
• Publication: Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174.

Backbone

• Vector backbone: pRGEN-CMV-CjCas9
• Backbone manufacturer: Addgene #89752
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dCjCas9
• Alt name: dCjeCas9
• Species: Synthetic; Campylobacter jejuni
• Mutation: D8A, H559A in cjCas9
• Promoter: CMV
• Tags / Fusion Proteins:
  • 10XGCN4 (N terminal on insert)
  • 2xSV40NLS (N terminal on insert)
  • SV40NLS-HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • 169912 pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D
• A portion of this plasmid was derived from a plasmid made by: 10X GCN4 was amplified from Addgene #60910

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry