CALID
(Plasmid
#169708)
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PurposeCre-activated light-inducible Dre recombinase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAdapted from px330
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namea double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
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SpeciesSynthetic
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Mutationnone
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CALID was a gift from Dali Li (Addgene plasmid # 169708 ; http://n2t.net/addgene:169708 ; RRID:Addgene_169708) -
For your References section:
Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Li H, Zhang Q, Gu Y, Wu Y, Wang Y, Wang L, Feng S, Hu Y, Zheng Y, Li Y, Ye H, Zhou B, Lin L, Liu M, Yang H, Li D. Proc Natl Acad Sci U S A. 2020 Dec 29;117(52):33426-33435. doi: 10.1073/pnas.2003991117. Epub 2020 Dec 14. 10.1073/pnas.2003991117 PubMed 33318209