pX20
(Plasmid
#169443)
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Purpose(Empty Backbone) Used for guide cloning with PCR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepX330
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX20 was a gift from Richard Huganir (Addgene plasmid # 169443 ; http://n2t.net/addgene:169443 ; RRID:Addgene_169443) -
For your References section:
An optimized CRISPR/Cas9 approach for precise genome editing in neurons. Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL. Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202. 10.7554/eLife.65202 PubMed 33689678