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pCMV6-Entry-EPHX1c.416A>G-Flag
(Plasmid #169170)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcmv entry
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    epoxide hydrolase 1
  • Alt name
    EPHX1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1461
  • Mutation
    c.337T>C
  • GenBank ID
    NM_000120
  • Entrez Gene
    EPHX1 (a.k.a. EPHX, EPOX, HYL1, MEH)
  • Promoter CMV
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site sgfI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer GGACTTTCCAAAATGTCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-Entry-EPHX1c.416A>G-Flag was a gift from Bruno Feve & Jeremie Gautheron (Addgene plasmid # 169170 ; http://n2t.net/addgene:169170 ; RRID:Addgene_169170)

  • For your References section:

    EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence. Gautheron J, Morisseau C, Chung WK, Zammouri J, Auclair M, Baujat G, Capel E, Moulin C, Wang Y, Yang J, Hammock BD, Cerame B, Phan F, Feve B, Vigouroux C, Andreelli F, Jeru I. Elife. 2021 Aug 3;10. pii: 68445. doi: 10.7554/eLife.68445. 10.7554/eLife.68445 PubMed 34342583
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