Skip to main content
Addgene

pCOLADuet-1_nsp10 (SARS-CoV-2)
(Plasmid #169158)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCOLADuet-1
  • Total vector size (bp) 4107
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nsp10
  • Alt name
    untagged nsp10
  • Alt name
    SARS-CoV-2 nsp10 cofactor
  • Species
    SARS-CoV-2
  • Mutation
    Codon optimised for E. coli
  • GenBank ID
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter T7
  • Tag / Fusion Protein
    • Methionine (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGCGACTCCTGCATTAG
  • 3′ sequencing primer ATGCGGCCGTGTACAATAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Unlike SARS-CoV-2 nsp10 that gets expressed in the context of a larger polypeptide, this SARS-CoV-2 nsp10 gets expressed from a 5' ATG, and thus might contain a methionine at the N-terminus that is not present in the nsp5-cleaved nsp10 in cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCOLADuet-1_nsp10 (SARS-CoV-2) was a gift from John Diffley (Addgene plasmid # 169158 ; http://n2t.net/addgene:169158 ; RRID:Addgene_169158)

  • For your References section:

    Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp14/nsp10 exoribonuclease. Canal B, McClure AW, Curran JF, Wu M, Ulferts R, Weissmann F, Zeng J, Bertolin AP, Milligan JC, Basu S, Drury LS, Deegan TD, Fujisawa R, Roberts EL, Basier C, Labib K, Beale R, Howell M, Diffley JFX. Biochem J. 2021 Jul 16;478(13):2445-2464. doi: 10.1042/BCJ20210198. 10.1042/BCJ20210198 PubMed 34198326
Related items:
Commonly requested with: