FLX1.8NFATGFPpd2HS4
(Plasmid #169096)

• Purpose: Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
• Depositing Lab: Geoffrey Owens
• Publication: FIV-based lentiviral NFAT reporter vector (unpublished)

Backbone

• Vector backbone: FL1.8WPREHS4
• Total vector size (bp): 6717
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: NFAT enhancer (from IL-4 promoter)
• Species: H. sapiens (human)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nsi I (not destroyed)
• 3′ cloning site: Bgl II (not destroyed)
• 5′ sequencing primer: -

Gene/Insert 2

• Gene/Insert name: Destabilized green fluorescent protein
• Alt name: EGFPpd2
• Species: Aequorea victoria
• Insert Size (bp): 855
• Promoter: NFAT enhancer + synthetic TATA box

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Mlu I (not destroyed)
• 3′ cloning site: Sal I (not destroyed)
• 5′ sequencing primer: -

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: NFAT ehancer was PCR amplified from 9X NFAT-apha-MHC-Luc (Addgene plasmid 5194)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry