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Addgene

XE58 Xwnt-5Amyc-CS2+
(Plasmid #16870)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-5Amyc-CS2+
  • Alt name
    wnt5
  • Alt name
    xwnt
  • Species
    X. laevis (frog)
  • Entrez Gene
    wnt5a.L (a.k.a. Xwnt-5A, wnt-5a, wnt5a-a, wnt5a-b)
  • Tag / Fusion Protein
    • myc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (destroyed during cloning)
  • 3′ cloning site BamH1 (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The coding region of contruct XE4 was isolated as a Bgl II insert and subcloned into
the BamHI site of CS2+.

Plasmid History:
Constructed by Julia Yang-Snyder (unpublished)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE58 Xwnt-5Amyc-CS2+ was a gift from Randall Moon (Addgene plasmid # 16870 ; http://n2t.net/addgene:16870 ; RRID:Addgene_16870)
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