pCAG roGFP2-mito
(Plasmid
#168509)
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PurposeExpression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168509 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemito-roGFP2
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SpeciesSynthetic
- Promoter CAG
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Tag
/ Fusion Protein
- roGFP2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG roGFP2-mito was a gift from Franck Polleux (Addgene plasmid # 168509 ; http://n2t.net/addgene:168509 ; RRID:Addgene_168509) -
For your References section:
MFF-dependent mitochondrial fission regulates presynaptic release and axon branching by limiting axonal mitochondria size. Lewis TL Jr, Kwon SK, Lee A, Shaw R, Polleux F. Nat Commun. 2018 Nov 27;9(1):5008. doi: 10.1038/s41467-018-07416-2. 10.1038/s41467-018-07416-2 PubMed 30479337
