Skip to main content
Addgene

pMRX-IBU-PFAS-FLAG
(Plasmid #168226)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMRX-IBU-FLAG
  • Backbone manufacturer
    Dr.Shoji Yamaoka of Tokyo Medical and Dental University
  • Backbone size w/o insert (bp) 6040
  • Total vector size (bp) 10055
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PFAS
  • Alt name
    FGAMS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4107
  • GenBank ID
    NM_012393
  • Entrez Gene
    PFAS (a.k.a. FGAMS, FGAR-AT, FGARAT, GATD8, PURL)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTTaaaGGATCCggGATGTCCCCAGTCCTTCACTTCTATG
  • 3′ sequencing primer ctgaaccagagcccGAATTCAAGCAGCTCCCTTCCAGGGTC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    It has the pMX backbone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IBU-PFAS-FLAG was a gift from Noboru Mizushima (Addgene plasmid # 168226 ; http://n2t.net/addgene:168226 ; RRID:Addgene_168226)

  • For your References section:

    Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy. Mimura K, Sakamaki JI, Morishita H, Kawazu M, Mano H, Mizushima N. J Biol Chem. 2021 Jan-Jun;296:100780. doi: 10.1016/j.jbc.2021.100780. Epub 2021 May 14. 10.1016/j.jbc.2021.100780 PubMed 34000301
Related items:
Commonly requested with: