pDEST(frt)(ATG_V5-APEX2_noSTOP)
(Plasmid
#168207)
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Purpose(Empty Backbone) Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFRT
- Backbone size (bp) 7721
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Vector typeMammalian Expression, Unspecified ; pDEST GATEWAY
- Promoter CMV
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- V5 APEX2 (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsAdditional Chloramphenicol resistance included with ccdB
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVf
- 3′ sequencing primer BGHr (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST(frt)(ATG_V5-APEX2_noSTOP) was a gift from Markus Hafner (Addgene plasmid # 168207 ; http://n2t.net/addgene:168207 ; RRID:Addgene_168207) -
For your References section:
Proximity-CLIP provides a snapshot of protein-occupied RNA elements in subcellular compartments. Benhalevy D, Anastasakis DG, Hafner M. Nat Methods. 2018 Dec;15(12):1074-1082. doi: 10.1038/s41592-018-0220-y. Epub 2018 Nov 26. 10.1038/s41592-018-0220-y PubMed 30478324
Map uploaded by the depositor.
