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Addgene

pAEMXT-eLACCO1.1
(Plasmid #167946)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAEMXT
  • Backbone manufacturer
    Covalys
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6825
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD59-eLACCO1.1-GPI
  • Species
    Synthetic
  • Insert Size (bp)
    1997
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer SV40
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAEMXT-eLACCO1.1 was a gift from Robert Campbell (Addgene plasmid # 167946 ; http://n2t.net/addgene:167946 ; RRID:Addgene_167946)

  • For your References section:

    A genetically encoded fluorescent biosensor for extracellular L-lactate. Nasu Y, Murphy-Royal C, Wen Y, Haidey JN, Molina RS, Aggarwal A, Zhang S, Kamijo Y, Paquet ME, Podgorski K, Drobizhev M, Bains JS, Lemieux MJ, Gordon GR, Campbell RE. Nat Commun. 2021 Dec 6;12(1):7058. doi: 10.1038/s41467-021-27332-2. 10.1038/s41467-021-27332-2 PubMed 34873165
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