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XE131 XFz1-CS2P+
(Plasmid #16793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XFz1-CS2P+
  • Alt name
    frizzled
  • Alt name
    fzd1
  • Species
    X. laevis (frog)
  • Entrez Gene
    fzd1 (a.k.a. Xfrizzled-1, frizzled1, frz-1, frz1, fzd1-A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: PCR amplified coding region of Xenopus Frizzled-1 in Eco-XbaI.
site of CS2+.
Linearize with Asp718 to make sense RNA.

Ref: J. Brown et al., Development, Growth and Differentiation (2000).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE131 XFz1-CS2P+ was a gift from Randall Moon (Addgene plasmid # 16793 ; http://n2t.net/addgene:16793 ; RRID:Addgene_16793)

  • For your References section:

    The maternal Xenopus beta-catenin signaling pathway, activated by frizzled homologs, induces goosecoid in a cell non-autonomous manner. Brown JD, Hallagan SE, McGrew LL, Miller JR, Moon RT. Dev Growth Differ. 2000 Aug. 42(4):347-57. 10.1046/j.1440-169x.2000.00517.x PubMed 10969734
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