pRecT_2
(Plasmid #167546)

• Purpose: Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
• Depositing Lab: Howard Hang
• Publication: Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 10.1128/AEM.00844-21.

Backbone

• Vector backbone: plZ12-Spec
• Backbone manufacturer: Luciano Marraffini
• Backbone size w/o insert (bp): 3729
• Total vector size (bp): 6411
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E. faecium Com15 recT under an IPTG inducible promoter, with lacI
• Insert Size (bp): 2896
• Promoter: IPTG

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTATTATAACATGTATTCACGAACG

Resource Information

• Supplemental Documents:
  • pRecT_2.gb
  • pRecT_2 plasmid map
• A portion of this plasmid was derived from a plasmid made by: IPTG promoter and lacI was taken from pPM145, a gift from Luciano Marraffini.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.09.01.278044v2 for BioRxiv preprint.