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Lenti-Dual-iCas9-EFS
(Plasmid #167501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lenti-iCas9-neo
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 15415
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4509
  • Promoter EFS
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-Dual-iCas9-EFS was a gift from Jake Liang (Addgene plasmid # 167501 ; http://n2t.net/addgene:167501 ; RRID:Addgene_167501)

  • For your References section:

    A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells. Park SB, Uchida T, Tilson S, Hu Z, Ma CD, Leek M, Eichner M, Hong SG, Liang TJ. Mol Ther Nucleic Acids. 2022 Apr 27;28:656-669. doi: 10.1016/j.omtn.2022.04.013. eCollection 2022 Jun 14. 10.1016/j.omtn.2022.04.013 PubMed 35615005
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