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Addgene

pMGIB-HA-hTCAB1
(Plasmid #167460)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167460 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMGIB
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7000
  • Modifications to backbone
    Retroviral backbone. Insert followed by IRES-Blasticidin cassette
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TCAB1
  • Alt name
    WDR79
  • Alt name
    WRAP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1647
  • GenBank ID
    NM_001143990.2
  • Entrez Gene
    WRAP53 (a.k.a. DKCB3, TCAB1, WDR79)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer IRES-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMGIB-HA-hTCAB1 was a gift from Steven Artandi (Addgene plasmid # 167460 ; http://n2t.net/addgene:167460 ; RRID:Addgene_167460)

  • For your References section:

    An Activity Switch in Human Telomerase Based on RNA Conformation and Shaped by TCAB1. Chen L, Roake CM, Freund A, Batista PJ, Tian S, Yin YA, Gajera CR, Lin S, Lee B, Pech MF, Venteicher AS, Das R, Chang HY, Artandi SE. Cell. 2018 Jun 28;174(1):218-230.e13. doi: 10.1016/j.cell.2018.04.039. Epub 2018 May 24. 10.1016/j.cell.2018.04.039 PubMed 29804836
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