XE107 XPKC alpha-GFP-CS2+
(Plasmid #16743)

• Depositing Lab: Randall Moon
• Publication: Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi: 10.1016/s0960-9822(99)80310-8.

Backbone

• Vector backbone: pCS2+
• Backbone manufacturer: RZPD
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKC-alpha
• Alt name: protein kinase c
• Alt name: prkc
• Species: X. laevis (frog)
• Entrez Gene: prkca (a.k.a. MGC82897, pkcalpha, pkc-alpha)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: Xenopus PKC alpha with Heim et al GFP variant at carboxy terminus. For transcription cut with Not 1 and use SP6.

Notes for Use:
Ref: Sheldahl et al., Current Biology 9:695-698, 1999.