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pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2
(Plasmid #167296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX458
  • Total vector size (bp) 9720
  • Modifications to backbone
    Inserted a second U6 gRNA expression cassette
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAX
  • gRNA/shRNA sequence
    GCTGCAGGATGATTGCCGCCG, GTCTGACGGCAACTTCAACTG
  • Species
    H. sapiens (human)
  • Entrez Gene
    BAX (a.k.a. BCL2L4)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2 was a gift from Agnel Sfeir (Addgene plasmid # 167296 ; http://n2t.net/addgene:167296 ; RRID:Addgene_167296)

  • For your References section:

    Nuclear sensing of breaks in mitochondrial DNA enhances immune surveillance. Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A. Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w. Epub 2021 Feb 24. 10.1038/s41586-021-03269-w PubMed 33627873
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