pMCSG53_Nsp3_Unique
(Plasmid
#167258)
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PurposeBacterial expression of Nsp3 Macrodomain 2 and Macrodomain 3 (residues 413-676)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167258 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepMCSG53
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNsp3 (residues 413-676)
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Alt nameSARS unique domain, SUD-N, SUD-M
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SpeciesSARS-CoV-2
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Mutationnone
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
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Tag
/ Fusion Protein
- His6-TEV site (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCSG53_Nsp3_Unique was a gift from Alexei Savchenko (Addgene plasmid # 167258 ; http://n2t.net/addgene:167258 ; RRID:Addgene_167258)
