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PurposeHelper/packaging plasmid for production of AAV2/11 pseudotype vector
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166888 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP72
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Backbone manufacturerPromega
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAV2 rep/AAV11 cap
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Alt nameAAV2/11
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SpeciesAdeno-associated virus
- Promoter AAV2 p5
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Adenovirus VA, E4, and E2a genes are also present on the backbone.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEEV2/11 was a gift from Tadahito Kanda & Seiichiro Mori (Addgene plasmid # 166888 ; http://n2t.net/addgene:166888 ; RRID:Addgene_166888) -
For your References section:
Two novel adeno-associated viruses from cynomolgus monkey: pseudotyping characterization of capsid protein. Mori S, Wang L, Takeuchi T, Kanda T. Virology. 2004 Dec 20;330(2):375-83. doi: 10.1016/j.virol.2004.10.012. 10.1016/j.virol.2004.10.012 PubMed 15567432