Skip to main content
Addgene

pCMV-LOx-IRES-EGFP
(Plasmid #166877)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 6203
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lactate oxidase
  • Alt name
    LOx
  • Species
    Synthetic
  • Insert Size (bp)
    1125
  • GenBank ID
    D50611.1
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer CMV forward: ATGGGCGGTAGGCGTGTA
  • 3′ sequencing primer IRES reverse: CCAAGTCAGTGGCTGCAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-LOx-IRES-EGFP was a gift from Sergey Kasparov (Addgene plasmid # 166877 ; http://n2t.net/addgene:166877 ; RRID:Addgene_166877)

  • For your References section:

    Reducing l-lactate release from hippocampal astrocytes by intracellular oxidation increases novelty induced activity in mice. Vaccari Cardoso B, Shevelkin AV, Terrillion C, Mychko O, Mosienko V, Kasparov S, Pletnikov MV, Teschemacher AG. Glia. 2021 Jan 5. doi: 10.1002/glia.23960. 10.1002/glia.23960 PubMed 33400321
Related items:
Commonly requested with: