XE101 alpha transducin CS2+
(Plasmid
#16681)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
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Backbone manufacturerRZPD
- Backbone size w/o insert (bp) 4095
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealpha-transducin
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Alt nametransducin
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Alt nameatrc
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SpeciesH. sapiens (human)
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Entrez GeneGNAT1 (a.k.a. CSNBAD3, GBT1, GNATR)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert: alpha-transducin from Jim Hurley, pBBIIAS. Linearize with Not1 and transcribe with SP6.
Ref: Slusarski et al., Nature 390:410-413, 1997.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE101 alpha transducin CS2+ was a gift from Randall Moon (Addgene plasmid # 16681 ; http://n2t.net/addgene:16681 ; RRID:Addgene_16681) -
For your References section:
Interaction of Wnt and a Frizzled homologue triggers G-protein-linked phosphatidylinositol signalling. Slusarski DC, Corces VG, Moon RT. Nature. 1997 Nov 27;390(6658):410-3. doi: 10.1038/37138. 10.1038/37138 PubMed 9389482