pcDNA3.1 LIC 6A HIS-TEV-DPP9S
(Plasmid
#166791)
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PurposeMammalian cell expression vector for His-tagged DPP9S
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166791 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1 LIC 6A
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDPP9
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Alt nameDPP9S
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SpeciesH. sapiens (human)
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Entrez GeneDPP9 (a.k.a. DP9, DPLP9, DPP IX, DPRP-2, DPRP2, HATIS, IMD111)
- Promoter CMV
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Tag
/ Fusion Protein
- HIS-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV F
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mammalian cell expression vector for His-tagged DPP9S
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 LIC 6A HIS-TEV-DPP9S was a gift from Hao Wu (Addgene plasmid # 166791 ; http://n2t.net/addgene:166791 ; RRID:Addgene_166791) -
For your References section:
DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation. Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H. Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 10.1038/s41586-021-03350-4. 10.1038/s41586-021-03350-4 PubMed 33731932
