DNMT1 KO Neo
(Plasmid
#16649)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 16649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSL301
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3264
-
Vector typeBacterial Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDNMT1 knock-out construct
-
Alt nameDNMT1
-
SpeciesH. sapiens (human)
-
Entrez GeneDNMT1 (a.k.a. ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' arm is 4.3 kb. 3' arm is 3.0 kb. See author's map for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
DNMT1 KO Neo was a gift from Bert Vogelstein (Addgene plasmid # 16649 ; http://n2t.net/addgene:16649 ; RRID:Addgene_16649) -
For your References section:
DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Rhee I, Bachman KE, Park BH, Jair KW, Yen RW, Schuebel KE, Cui H, Feinberg AP, Lengauer C, Kinzler KW, Baylin SB, Vogelstein B. Nature. 2002 Apr 4. 416(6880):552-6. 10.1038/416552a PubMed 11932749
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/48/16/1b3f1768-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)