pBS L30 mRuby3-Rab27A
(Plasmid #166432)

• Purpose: Rab27A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
• Depositing Lab: Raphael Gaudin
• Publication: Generation of mRuby3-fused Rab GTPases constructs (unpublished)

Backbone

• Vector backbone: pBS
• Modifications to backbone: Modified for mammalian expression using the promoter of the mouse ribosomal protein L30 gene ("weak promoter") and a HSV TK polyA tail
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rab27A
• Species: H. sapiens (human)
• GenBank ID: 5873 NC_000015.10
• Promoter: L30 weak promoter
• Tag / Fusion Protein:
  • mRuby3 (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry