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Addgene

pdCas9-sgRNA-RFP
(Plasmid #166005)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166005 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCas9-CR4
  • Backbone size (bp) 1700
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Tight inducible control of dCas9 and sgRNA expression in bacteria. sgRNA spacer is cloned in between BsaI sites, replacing an RFP cassette to facilitate identification of successful clones.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdCas9-sgRNA-RFP was a gift from Martin Buck (Addgene plasmid # 166005 ; http://n2t.net/addgene:166005 ; RRID:Addgene_166005)

  • For your References section:

    An easy-to-use CRISPRi plasmid tool for inducible knockdown in E. coli. Bradley RW. Biotechnol Rep (Amst). 2021 Oct 9;32:e00680. doi: 10.1016/j.btre.2021.e00680. eCollection 2021 Dec. 10.1016/j.btre.2021.e00680 PubMed 34703773
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