pET11b-SpyCatcher-HA2
(Plasmid #165991)

• Purpose: SpyCatcher-fused influenza HA ministem with removable HisTag
• Depositing Lab: Vesa Hytönen
• Publication: Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):25. doi: 10.1186/s12951-021-00772-0.

Backbone

• Vector backbone: pET-11b
• Backbone size w/o insert (bp): 5676
• Total vector size (bp): 6435
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyCatcher-HA2
• Alt name: H1F
• Alt name: Influenza hemagglutinin ministem
• Species: Synthetic
• Insert Size (bp): 843
• Promoter: T7
• Tags / Fusion Proteins:
  • HisTag (N terminal on insert)
  • SpyCatcher (N terminal on insert)
  • Foldon (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BlpI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pET11b-SpyCatcher-H1F.gbk
• A portion of this plasmid was derived from a plasmid made by: The gene was syntesized and cloned by Genscript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original publications:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.

HA ministem:
Mallajosyula VV, Citron M, Ferrara F, Lu X, Callahan C, Heidecker GJ, Sarma SP, Flynn JA, Temperton NJ, Liang X, Varadarajan R. Influenza hemagglutinin stem-fragment immunogen elicits broadly neutralizing antibodies and confers heterologous protection. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):E2514-23. doi: 10.1073/pnas.1402766111. Epub 2014 Jun 9. PMID: 24927560; PMCID: PMC4078824.