BAX KO vector
(Plasmid
#16575)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBAX knockout
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Alt nameBAX
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SpeciesH. sapiens (human)
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Insert Size (bp)6000
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Entrez GeneBAX (a.k.a. BCL2L4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This targeting construct consists of two homologous arms and a hygromycin-resistant gene.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BAX KO vector was a gift from Bert Vogelstein (Addgene plasmid # 16575 ; http://n2t.net/addgene:16575 ; RRID:Addgene_16575) -
For your References section:
Role of BAX in the apoptotic response to anticancer agents. Zhang L, Yu J, Park BH, Kinzler KW, Vogelstein B. Science. 2000 Nov 3. 290(5493):989-92. 10.1126/science.290.5493.989 PubMed 11062132
Map uploaded by the depositor.
