pGST-PPARgamma
(Plasmid #16549)

• Depositing Lab: Bert Vogelstein
• Publication: He et al Cell. 1999 Oct 29. 99(3):335-45.

Backbone

• Vector backbone: pGEX-2TK
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPAR gamma
• Species: H. sapiens (human)
• Insert Size (bp): 744
• Mutation: N-terminal DNA-binding domain: aa 1-248
• Entrez Gene: PPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that there are some discrepancies between the insert in this plasmid and the canonical GenBank PPARgamma. The Vogelstein lab maintains that this plasmid worked as specified when they made and used it.