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pAAV-CMV-mCherry-NLS
(Plasmid #165442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CMV-EGFP-NLS
  • Backbone manufacturer
    Esteban Engel lab
  • Total vector size (bp) 5121
  • Modifications to backbone
    Replaced EGFP with mCherry
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-NLS
  • Insert Size (bp)
    804
  • Promoter CMV
  • Tag / Fusion Protein
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer agaGCTAGCatggtgagcaagggcgagg
  • 3′ sequencing primer acaCTCGAGgcttgtacagctcgtccatgcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-mCherry-NLS was a gift from Esteban Engel (Addgene plasmid # 165442 ; http://n2t.net/addgene:165442 ; RRID:Addgene_165442)

  • For your References section:

    Novel tool to quantify with single-cell resolution the number of incoming AAV genomes co-expressed in the mouse nervous system. Maturana CJ, Verpeut JL, Kooshkbaghi M, Engel EA. Gene Ther. 2021 Jun 28. pii: 10.1038/s41434-021-00272-8. doi: 10.1038/s41434-021-00272-8. 10.1038/s41434-021-00272-8 PubMed 34176926
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