pGGG Wheat GUS marker
(Plasmid #165418)

• Purpose: Wheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter
• Depositing Labs: Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
• Publication: Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.1002/cpz1.58.

Backbone

• Vector backbone: pGoldenGreenGate (pGGG) (pGreenII based Golden Gate compatible vector with additional LB)
• Vector type: Wheat transformation binary vector
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Os Ubiquitin Promoter::Gus (2x introns)::NosT
• Alt name: RUbiP::GUS2int::NosT
• Species: Synthetic
• Insert Size (bp): 5732
• Promoter: OS UbiP::GUS (2xint)::NosT

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Golden Gate BpiI (destroyed during cloning)
• 3′ cloning site: Golden Gate BpiI (destroyed during cloning)
• 5′ sequencing primer: Actin P Forward 5’-CGTATCAAAGTACCGACAAAAACA-3’
• 3′ sequencing primer: RB-R1 Reverse 5’-CCCGCCAATATATCCTGTCA-3’

Resource Information

• Supplemental Documents:
  • pGGG Wheat GUS marker.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry