FBE/SBE-Luc
(Plasmid
#16525)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16525 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBV-Luc
-
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
-
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameFBE/SBE
-
Alt nameFAST-1 binding element
-
Alt nameSmad binding element
-
Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
FBE/SBE-luc was made by cloning the SBE-containing oligonucleotide into the Sac1 and Nhe1 sites of FBE-luc.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FBE/SBE-Luc was a gift from Bert Vogelstein (Addgene plasmid # 16525 ; http://n2t.net/addgene:16525 ; RRID:Addgene_16525) -
For your References section:
Characterization of human FAST-1, a TGF beta and activin signal transducer. Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B. Mol Cell. 1998 Jul . 2(1):121-7. 10.1016/S1097-2765(00)80120-3 PubMed 9702198
Map uploaded by the depositor.
