CMV-Fast-1 wild-type
(Plasmid #16521)

• Depositing Lab: Bert Vogelstein
• Publication: Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

Backbone

• Vector backbone: pCI-Neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FAST-1
• Alt name: Fast1
• Alt name: forkhead activin signal transducer-1
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Entrez Gene: FOXH1 (a.k.a. FAST-1, FAST1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo.