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Addgene

GFP-HA-BACE1
(Plasmid #165032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Total vector size (bp) 6239
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BACE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1506
  • GenBank ID
    AF201468.1
  • Entrez Gene
    BACE1 (a.k.a. ASP2, BACE, HSPC104)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (embedded after propeptide cleavage site) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV forwqrd
  • 3′ sequencing primer SV40pA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-HA-BACE1 was a gift from Juan Bonifacino (Addgene plasmid # 165032 ; http://n2t.net/addgene:165032 ; RRID:Addgene_165032)

  • For your References section:

    Adaptor protein 2-mediated endocytosis of the beta-secretase BACE1 is dispensable for amyloid precursor protein processing. Prabhu Y, Burgos PV, Schindler C, Farias GG, Magadan JG, Bonifacino JS. Mol Biol Cell. 2012 Jun;23(12):2339-51. doi: 10.1091/mbc.E11-11-0944. Epub 2012 May 2. 10.1091/mbc.E11-11-0944 PubMed 22553349
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