pmiHiFiCas9
(Plasmid #164832)

• Purpose: miHiFiCas9 expressing plasmid
• Depositing Lab: Eugene Chen
• Publication: Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi: 10.1038/s41467-020-19842-2.

Backbone

• Vector backbone: pJDS246
• Backbone size w/o insert (bp): 2466
• Total vector size (bp): 7722
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miHiFiCas9 (contains Brex27 at sequence 5031-5138).
• Species: Synthetic; Streptococcus pyogenes
• Insert Size (bp): 5387
• Mutation: change R 691 to A
• Promoter: CMV
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pmiHiFiCas9
• A portion of this plasmid was derived from a plasmid made by: The original gene was from Addgene plasmid 47754

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry