lenti SYN-SVI-dCas9-VPR
(Plasmid
#164575)
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PurposeExpresses an intron-containing dCas9-VPR fusion driven by human SYN promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelentiCRISPR v2
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Backbone manufacturerFeng Zhang Addgene plasmid #52961
- Backbone size w/o insert (bp) 7546
- Total vector size (bp) 13968
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Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLAG-dCas9-VPR containing an SV40 intron
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SpeciesSynthetic
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Insert Size (bp)6422
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lenti SYN-SVI-dCas9-VPR was a gift from Jeremy Day (Addgene plasmid # 164575 ; http://n2t.net/addgene:164575 ; RRID:Addgene_164575) -
For your References section:
A Cre-dependent CRISPR/dCas9 system for gene expression regulation in neurons. Carullo NVN, Hinds JE, Revanna JS, Tuscher JJ, Bauman AJ, Day JJ. eNeuro. 2021 Jul 26. pii: ENEURO.0188-21.2021. doi: 10.1523/ENEURO.0188-21.2021. 10.1523/ENEURO.0188-21.2021 PubMed 34321217
Map uploaded by the depositor.
