pDiCre-mCherry
(Plasmid #164572)

• Purpose: Integration of a DiCre cassette in PbGFP parasite genome
• Depositing Lab: Olivier Silvie
• Publication: Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. doi: 10.1371/journal.pone.0236616. eCollection 2020.

Backbone

• Vector backbone: pBluescript
• Vector type: Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: FKBP12-CRE59
• Alt name: FKBP12-linker peptide 2-Cre(19-59)
• Species: Synthetic
• Insert Size (bp): 516
• Promoter: Bidirectional PbEF1alpha promoter (PBANKA_1133400)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: FRB-CRE60
• Alt name: FRB-linker peptide 2- Cre(60-343)
• Species: Synthetic
• Insert Size (bp): 1200
• Promoter: Bidirectional PbEF1alpha promoter (PBANKA_1133300)

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 731
• Promoter: Truncated PbHSP70 promoter (PBANKA_0711900)

Cloning Information for Gene/Insert 3

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Gene/Insert 4

• Gene/Insert name: Bifunctional dihydrofolate reductase-thymidylate synthase
• Species: Toxoplasma gondii
• Insert Size (bp): 1801
• Entrez Gene: TGME49_249180 (a.k.a. TGME49_249180, TGME49_049180)
• Promoter: Bidirectional PbEF1alpha promoter (PBANKA_1133400)

Cloning Information for Gene/Insert 4

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pDiCre-mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry