PG13-luc (wt p53 binding sites)
(Plasmid #16442)

• Purpose: Luciferase reporter containing 13 copies of the p53-binding consensus sequence
• Depositing Lab: Bert Vogelstein
• Publication: el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Backbone

• Vector backbone: pBluescript II SK + luc
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p53-binding site
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 45 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PG13-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.

PG13 contains 13 copies of the p53-binding consensus sequence.

Direct repeats of the oligonucleotide PG (5'-CCAGGCAAGTCCAGGCAGG-3') were used for the
p53 binding sequences.