pMTP130 (pTA106_TnsABC(Tn6900))
(Plasmid #164261)

• Purpose: Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.
• Depositing Lab: Joseph Peters
• Publication: Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31464-1. doi: 10.1016/j.cell.2020.11.005.

Backbone

• Vector backbone: pTA106
• Backbone size w/o insert (bp): 4217
• Total vector size (bp): 7347
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
• Species: Aeromonas salmonicida
• Insert Size (bp): 3522
• Mutation: (tnsA) Changed Alanine 125 to Aspartic acid
• GenBank ID: NZ_CP022176.1
• Promoter: Lactose

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GCGCCCAATACGCAAACCGCCTCTC

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSC101 replicon