pBS-mSema3C
(Plasmid
#16426)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeIn Situ Template
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepBS-Sema3C
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Alt nameSemaphorin 3C
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SpeciesM. musculus (mouse)
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Insert Size (bp)3200
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MutationNone
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Entrez GeneSema3c (a.k.a. 1110036B02Rik, SemE, Semae)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJonathan A. Raper, University of Pennsylvania
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Antisense probes are generated by digesting with NotI and transcribing with T3 RNA Polymerase.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-mSema3C was a gift from Jonathan Raper (Addgene plasmid # 16426 ; http://n2t.net/addgene:16426 ; RRID:Addgene_16426)