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Addgene

GLI-3 bs-2
(Plasmid #16420)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16420 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLI3
  • Alt name
    Gli-3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5085
  • Entrez Gene
    GLI3 (a.k.a. ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SstI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GLI3 bs-2 contains the entire coding region of Gli-3 (5085bp) cloned into the SstI/SalI site of pBluescript SK.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GLI-3 bs-2 was a gift from Bert Vogelstein (Addgene plasmid # 16420 ; http://n2t.net/addgene:16420 ; RRID:Addgene_16420)

  • For your References section:

    The GLI gene is a member of the Kruppel family of zinc finger proteins. Kinzler KW, Ruppert JM, Bigner SH, Vogelstein B. Nature. 1988 Mar 24. 332(6162):371-4. 10.1038/332371a0 PubMed 2832761
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