GLI K12
(Plasmid #16419)

• Depositing Lab: Bert Vogelstein
• Publication: Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Backbone

• Vector backbone: pBluescript KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GLI
• Alt name: Gli-1
• Alt name: glioma-associated oncogene homolog 1
• Species: H. sapiens (human)
• Insert Size (bp): 3601
• Entrez Gene: GLI1 (a.k.a. GLI, PAPA8, PPD1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GlI K12 contains the entire coding region of GLI (-79 to 3522) cloned into the EcoRI site of pBluescript KS. The GLI cDNA contains an internal EcoRI site so the insert should be excised by cleavage with HindIII (at 5' end of Gli cDNA) and XbaI (at 3' end of Gli cDNA).