Human mTORC1 Focused Library
(Pooled Library #164084)

• Purpose:

  This lentiviral CRISPR library targets human genes required for mTORC1 activation and inhibition with 10 sgRNAs per gene.

• Vector Backbone:

  lentiCRISPR v1 (Plasmid #49535) - expresses Cas9

  Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages recipient scientists to use the updated lentiCRISPR v2 (Plasmid #52961), which can be requested separately.

• Depositing Labs: David Sabatini
• Publication: Condon et al PNAS. 2021 Jan 26. doi: 10.1073/pnas.2022120118

Library Details

• Species: Human
• Genes targeted: 712
• gRNAs: 7,501
• Controls:
  246 targeting control sgRNA
  129 intergenic control sgRNA
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Transformation Protocol (DOCX, 15 KB)
  • PCR Protocol for Illumina Sequencing Preparation (DOCX, 14 KB)
• Depositor Data:
  • List of Targeted Genes and gRNA Sequences (CSV, 327 KB)
  • NGS Read Count Data (TXT, 123 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

• Sabatini Lab mTORC1 Focused Library

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms for analysis.

Please visit https://doi.org/10.1101/2020.10.22.351361 for bioRxiv preprint.