pLEX307 RFP
(Plasmid
#163976)
-
PurposeMammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 163976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLEX307
-
Backbone manufacturerAddgene #41392
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRFP
-
SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLEX307 RFP was a gift from Daniel Bachovchin (Addgene plasmid # 163976 ; http://n2t.net/addgene:163976 ; RRID:Addgene_163976) -
For your References section:
Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes. Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H. Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y. 10.1038/s41467-020-20320-y PubMed 33420033