PKC delta DN
(Plasmid #16389)

• Depositing Lab: Bernard Weinstein
• Publication: Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-16.

Backbone

• Vector backbone: pHACE
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKC delta isoform 2
• Species: M. musculus (mouse)
• Insert Size (bp): 2022
• Mutation: Dominant negative (kinase inactive): K376R
• Entrez Gene: Prkcd (a.k.a. D14Ertd420e, PKC[d], PKCdelta, Pkcd)
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry