pAAV-hSyn-DIO-hMECP2-cycle3GFP
(Plasmid #163706)

• Purpose: pAAV plasmid for Cre-dependent expression of human MECP2 fused with cycle3 GFP under Syn promoter
• Depositing Lab: Michisuke Yuzaki
• Publication: Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766. doi: 10.1523/JNEUROSCI.1281-19.2020. Epub 2020 Oct 12.

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Backbone

• Vector backbone: pAAV
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hMECP2-cycle3GFP
• Species: H. sapiens (human); Aequorea victoria
• Entrez Gene: MECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)
• Promoter: hSyn

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GFP-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: hMecp2-cycle3GFP is from Huda Zoghbi (Addgene plasmid # 48078)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry