pVIPR2-ERT2CreERT2pA
(Plasmid
#163628)
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PurposeHeart-specific tamoxifen-dependent Cre expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II SK(+)
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase
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SpeciesBacteriophage P1
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Tag
/ Fusion Protein
- ERT2 on both N-terminal and C-terminal of Cre
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVIPR2-ERT2CreERT2pA was a gift from Daekee Lee (Addgene plasmid # 163628 ; http://n2t.net/addgene:163628 ; RRID:Addgene_163628) -
For your References section:
Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron. Chin HJ, Lee SY, Lee D. Lab Anim Res. 2020 Sep 15;36:31. doi: 10.1186/s42826-020-00065-x. eCollection 2020. 10.1186/s42826-020-00065-x PubMed 32983955