GSK-3beta-his (Xenopus)/pCS2+
(Plasmid
#16335)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeVertebrate expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameglycogen synthase kinase-3beta
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Alt nameGSK3b
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Alt nameGSK-3b
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Alt nameGSK-3b-his
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SpeciesX. laevis (frog)
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Insert Size (bp)1300
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MutationXenopus GSK-3 with 6X his tag
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Entrez Genegsk3b.L (a.k.a. XELAEV_180112401mg, gsk-3, gsk3, gsk3-beta, gsk3b, gsk3beta, xgsk-3)
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI ? (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
His tagged Xenopus GSK-3.
For sense transcripts, cut with NotI and transcribe with SP6
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GSK-3beta-his (Xenopus)/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16335 ; http://n2t.net/addgene:16335 ; RRID:Addgene_16335)