GSK-3beta S9A (Human)/pCS2+
(Plasmid
#16334)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16334 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeVertebrate expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameglycogen synthase kinase-3 beta
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Alt nameGSK3b
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Alt nameGSK-3beta
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Alt nameS9A, NPt
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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MutationGSK-3 beta with serine-9 to alanine point mutation
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Entrez GeneGSK3B
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GSK-3beta with ser9->ala mutation blocks N terminal phosphorylation. For sense transcripts, cut with NotI and transcribe with SP6.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GSK-3beta S9A (Human)/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16334 ; http://n2t.net/addgene:16334 ; RRID:Addgene_16334)